The discovery of glutathione first was in 1888 by De-Rey-Pailhade, but its role as an antioxidant and detoxifier was not discovered until 30 years later. Glutathione was first isolated by Sir Frederick Gowland Hopkins in 1926. Isolating glutathione lead to understanding of this powerful tripeptide and exploration of its many uses. Hopkins made is the discovery and characterization of glutathione that is described in the Journal of Biological Chemistry. He recognized that glutathione was important as a hydrogen acceptor in a number of biochemical reactions. In addition, Hopkins observed the way the body utilized oxygen in relation to glutathione levels.